Journal: Journal of experimental & clinical cancer research : CR

Article Title: Growth differentiation factor 15 contributes to cancer-associated fibroblasts-mediated chemo-protection of AML cells.

doi: 10.1186/s13046-016-0405-0

Figure Lengend Snippet: Fig. 4 GDF15 contributed to the CAF-mediated chemo-protection of the AML cells. (a-c) Western blot, ELISA and Quantitative RT-PCR analyses represented GDF15 expression in the MSC and the CAF cells. d The bar plots showed the relative viability of the THP-1 cells cultured in medium alone or co-cultured with CAFs added with or without an anti-GDF15 antibody under the treatment of Ara-c (10uM) for 48 h. e Quantitative RT- PCR showing GDF15 expression in the CAFs after nucleofection of a GDF15-specific siRNA or a non-targeting control siRNA for 48 h. f The number of viable THP-1 cells cultured in medium alone or direct co-cultured with the layer of CAFs after nucleofection of GDF15-siRNA or not under the treatment of Ara-C (10uM) for 48 h. The data are the result of three independent experiments and are presented as means ± SD,*p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: The rabbit anti-FSP1/S100A4 monoclonal antibody (Millipore, Billerica, MA, USA), rabbit anti-FAP polyclonal antibody (Abnova, Taipei, Taiwan), goat anti-α-SMA polyclonal antibody (Abcam, Cambridge, MA, USA), rabbit anti-GDF15 monoclonal antibody (Cell Signaling, Danvers, MA, USA), mouse antiVimentin monoclonal antibody (Santa Cruz, Dallas, CA, USA), and mouse anti-CD68 monoclonal antibody (Abcam) incubations were performed according to the manufacturer’s instructions.

Techniques: Western Blot, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Expressing, Cell Culture, Control

Journal: Journal of experimental & clinical cancer research : CR

Article Title: Growth differentiation factor 15 contributes to cancer-associated fibroblasts-mediated chemo-protection of AML cells.

doi: 10.1186/s13046-016-0405-0

Figure Lengend Snippet: Fig. 5 GDF15 expressed in the BM of AML in vivo. a ELISA analysis of GDF15 levels in the BM aspirates of the controls (n = 11) and treatment- naïve AML-CR (n = 9) and AML-refractory patients (n = 8) *** p < 0.001vs the control group. b Examples of immunohistochemistry for GDF15 in the BM sections of the normal control and the primary AML patient. Both images are at a magnification of 400×. c Immunohistochemistry identifies cells that express FAP (arrows) and GDF15 (arrowheads) in the AML patients. GDF15 could also be detected in the leukemia cells (triangle). The images on the left are at a magnification of 400× and on the right are at a magnification of 1000×

Article Snippet: The rabbit anti-FSP1/S100A4 monoclonal antibody (Millipore, Billerica, MA, USA), rabbit anti-FAP polyclonal antibody (Abnova, Taipei, Taiwan), goat anti-α-SMA polyclonal antibody (Abcam, Cambridge, MA, USA), rabbit anti-GDF15 monoclonal antibody (Cell Signaling, Danvers, MA, USA), mouse antiVimentin monoclonal antibody (Santa Cruz, Dallas, CA, USA), and mouse anti-CD68 monoclonal antibody (Abcam) incubations were performed according to the manufacturer’s instructions.

Techniques: In Vivo, Enzyme-linked Immunosorbent Assay, Control, Immunohistochemistry

Journal: Communications Biology

Article Title: Melatonin affects trophoblast epithelial-to-mesenchymal transition and oxidative damage resistance by modulating GDF15 expression to promote embryo implantation

doi: 10.1038/s42003-025-07834-1

Figure Lengend Snippet: After HTR-8/SVneo cells treated with different concentrations of melatonin for 24 h, the protein expression of GDF15, SMAD1, SMAD5, SMAD2/3, p-SMAD3, p-SMAD1/5, E-CAD, and N-CAD was detected ( A ). The protein expression of GDF15, SMAD2/3, p-SMAD3, E-CAD and N-CAD was detected after GDF15 knockdown treatment ( B ). After knocking down the expression of GDF15, melatonin (10 μM) was added again for 24 h to detect the protein expression of GDF15, SMAD2/3, p-SMAD3, E-CAD, and N-CAD ( C ). SIS3 (5 μM) was used for 6 h to inhibit SMAD3 protein phosphorylation and then add melatonin (10 μM) again for another 24 h to detect the protein expression of SMAD2/3, p-smad3, E-CAD and N-CAD ( D ). B-ACTIN/GAPDH is the internal reference protein. HTR-8/SVneo was cultured in a low-attachment 96-well plate for 24 h to form cell spheroids and co-cultured with ishikawa ( E ), and analyze the number of trophoblast cell spheres planted in each group at 0.5 h and 2 h ( F , G ). Transwell migration assay was used to detect the migration ability of cells in each group ( H ), and statistically analyzed the number of migrating cells in each group ( I , J ). Scale bar is 50 μm. Ns indicates no statistical significance, * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001.

Article Snippet: GDF15 (27455-1-AP, anti-rabbit, Proteintech, Wuhan, China, 1:1000), E-CAD (20874-1-AP, anti-rabbit, Proteintech, 1:1000), N-CAD (22018-1-AP, anti-rabbit, Proteintech, 1:1000), GPX4 (381958, Zen-bioscience, Chengdu, China 1:1000), SMAD2/3 (8685, anti-rabbit, Cell signaling technology, MA, USA, 1:1000), SMAD1 (6944, anti-rabbit, Cell signaling technology, 1:1000), SMAD5 (12534, anti-rabbit, Cell signaling technology, 1:1000), p-SMAD3 (9520, anti-rabbit, Cell signaling technology, 1:1000), p-SMAD1/5 (13820, anti-rabbit, Cell signaling technology, 1:1000), B-ACTIN (AP0060, anti-rabbit, Bioworld, Nanjing, China, 1:8000), GAPDH (AP0063, anti-rabbit, Bioworld, 1:8000).

Techniques: Expressing, Knockdown, Phospho-proteomics, Cell Culture, Transwell Migration Assay, Migration

Journal: Communications Biology

Article Title: Melatonin affects trophoblast epithelial-to-mesenchymal transition and oxidative damage resistance by modulating GDF15 expression to promote embryo implantation

doi: 10.1038/s42003-025-07834-1

Figure Lengend Snippet: Melatonin (10 mg/kg) and luzindole (1 mg/kg) were injected intraperitoneally into the mice on the day the plug was seen for 7 days. The uterus was harvested at E7.5 and the embryos were collected. Observe the embryo implantation sites of mice in each group ( A ), and perform statistical analysis on the number of uterine implantation sites ( B ). Immunohistochemical stain was used to analyze the localization and expression of GDF15, N-CAD and E-CAD in E7.5 embryonic and decidual tissues of mice in each group ( C ). E7.5 mouse embryos from each group were obtained and the protein expression levels of GPX4, GDF15, p-SMAD3, SMAD2/3, N-CAD and E-CAD were detected ( D ) B-ACTIN was the internal reference protein. Ns indicates no statistical significance, * indicates p < 0.05, ** indicates p < 0.01.

Article Snippet: GDF15 (27455-1-AP, anti-rabbit, Proteintech, Wuhan, China, 1:1000), E-CAD (20874-1-AP, anti-rabbit, Proteintech, 1:1000), N-CAD (22018-1-AP, anti-rabbit, Proteintech, 1:1000), GPX4 (381958, Zen-bioscience, Chengdu, China 1:1000), SMAD2/3 (8685, anti-rabbit, Cell signaling technology, MA, USA, 1:1000), SMAD1 (6944, anti-rabbit, Cell signaling technology, 1:1000), SMAD5 (12534, anti-rabbit, Cell signaling technology, 1:1000), p-SMAD3 (9520, anti-rabbit, Cell signaling technology, 1:1000), p-SMAD1/5 (13820, anti-rabbit, Cell signaling technology, 1:1000), B-ACTIN (AP0060, anti-rabbit, Bioworld, Nanjing, China, 1:8000), GAPDH (AP0063, anti-rabbit, Bioworld, 1:8000).

Techniques: Injection, Immunohistochemical staining, Staining, Expressing

Journal: Communications Biology

Article Title: Melatonin affects trophoblast epithelial-to-mesenchymal transition and oxidative damage resistance by modulating GDF15 expression to promote embryo implantation

doi: 10.1038/s42003-025-07834-1

Figure Lengend Snippet: The protein expression levels of GPX4 was detected after HTR-8/SVneo cells were treated with melatonin (10 μM) for 24 h ( A ). The protein expression of GPX4 was detected after the treatment of si-GDF15 ( B ) and the co-treatment of melatonin and si-GDF5 ( C ). B-ACTIN/GAPDH was an internal protein. H 2 O 2 (800 μM, 2 h) was used to induce oxidative stress in HTR-8/SVneo cells, and the intracellular GSH level after melatonin treatment was detected ( D ). After knocking down GDF15, GSH levels in HTR-8/SVneo cells were detected after melatonin treatment for 24 h ( E ). Fluorescent stain was used to detect the effects of melatonin and GDF15 on ROS levels in HTR-8/SVneo cells ( F , G ). Blue fluorescence represents the nuclear signal, and green represents the ROS signal. Fluorescent stain was used to detect the effects of melatonin and GDF15 on mitochondrial membrane potential in HTR-8/SVneo cells ( H – K ). The red fluorescence is JC1 monomer, and the green signal is JC1 monomer. The morphology of mitochondria in each group was observed using transmission electron microscopy ( L , M ). Black arrows represent mitochondrial swelling and disappearance of mitochondrial cristae, and red arrows represent mitochondrial membrane rupture. Flow cytometry was used to detect the degree of cell apoptosis ( N ), and the apoptosis rate of each group was calculated ( O ), where apoptotic cells = early apoptotic cells and mid-stage apoptotic cells. * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, and **** indicates p < 0.0001.

Article Snippet: GDF15 (27455-1-AP, anti-rabbit, Proteintech, Wuhan, China, 1:1000), E-CAD (20874-1-AP, anti-rabbit, Proteintech, 1:1000), N-CAD (22018-1-AP, anti-rabbit, Proteintech, 1:1000), GPX4 (381958, Zen-bioscience, Chengdu, China 1:1000), SMAD2/3 (8685, anti-rabbit, Cell signaling technology, MA, USA, 1:1000), SMAD1 (6944, anti-rabbit, Cell signaling technology, 1:1000), SMAD5 (12534, anti-rabbit, Cell signaling technology, 1:1000), p-SMAD3 (9520, anti-rabbit, Cell signaling technology, 1:1000), p-SMAD1/5 (13820, anti-rabbit, Cell signaling technology, 1:1000), B-ACTIN (AP0060, anti-rabbit, Bioworld, Nanjing, China, 1:8000), GAPDH (AP0063, anti-rabbit, Bioworld, 1:8000).

Techniques: Expressing, Staining, Fluorescence, Membrane, Transmission Assay, Electron Microscopy, Flow Cytometry

Journal: PLoS ONE

Article Title: Reconstitution of TGFBR2-Mediated Signaling Causes Upregulation of GDF-15 in HCT116 Colorectal Cancer Cells

doi: 10.1371/journal.pone.0131506

Figure Lengend Snippet: (A) Upregulated transcript levels of GDF-15 in the presence of Dox using real-time qRT-PCR analysis. (B) Western blot analysis of GDF-15 protein expression in total protein lysates (30 μg) of TGF-ß1 stimulated cells (4 h). Expression of GDF-15 is increased when TGFBR2 is expressed (+Dox) in both cell clones but not in the parental Tet-On cell line. TGF-ß1 signaling is indicated by increased pSmad2 levels in the TGFBR2-expressing clones. Addition of cycloheximide (CHX) reduces the expression level of GDF-15. ß-Actin and Smad2 served as a loading control.

Article Snippet: Primary antibodies were used as follows: mouse anti-TGFBR2 (sc-17799; Santa Cruz, Dallas, USA; 1:500, 4°C, overnight); mouse anti-ß-Actin (clone 4; MP Biomedicals, Solon, USA; 1:20,000, RT, 30 min); rabbit anti-GDF-15 (HPA011191; Sigma, Taufkirchen Germany; 1:500, 4°C, overnight); rabbit anti-phospho-Smad2 and rabbit anti-Smad2 (Ser465/467) and (86F7); Cell Signaling, Danvers, USA; 1:1000, 4°C, overnight).

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Clone Assay

Journal: PLoS ONE

Article Title: Reconstitution of TGFBR2-Mediated Signaling Causes Upregulation of GDF-15 in HCT116 Colorectal Cancer Cells

doi: 10.1371/journal.pone.0131506

Figure Lengend Snippet: [ 35 S]-L-methionine was used for metabolic labeling and subsequent immunoprecipitation of intracellular GDF-15 from total lysates (A) or secreted GDF-15 from cell culture medium (B). Cells were stimulated with TGF-ß1 for 4 h in (A) and 24 h in (B).

Article Snippet: Primary antibodies were used as follows: mouse anti-TGFBR2 (sc-17799; Santa Cruz, Dallas, USA; 1:500, 4°C, overnight); mouse anti-ß-Actin (clone 4; MP Biomedicals, Solon, USA; 1:20,000, RT, 30 min); rabbit anti-GDF-15 (HPA011191; Sigma, Taufkirchen Germany; 1:500, 4°C, overnight); rabbit anti-phospho-Smad2 and rabbit anti-Smad2 (Ser465/467) and (86F7); Cell Signaling, Danvers, USA; 1:1000, 4°C, overnight).

Techniques: Labeling, Immunoprecipitation, Cell Culture

Journal: PLoS ONE

Article Title: Reconstitution of TGFBR2-Mediated Signaling Causes Upregulation of GDF-15 in HCT116 Colorectal Cancer Cells

doi: 10.1371/journal.pone.0131506

Figure Lengend Snippet: (A) Proliferation assay is displayed as an average of six replicates. (B) Western blot analysis of TGFBR2-dependent pSmad2 levels. HCT116-TGFBR2 #5 cells were treated in absence and presence of human recombinant TGF-ß1 (10 ng/ml) and GDF-15 for 1 h. Smad2 served as an internal loading control.

Article Snippet: Primary antibodies were used as follows: mouse anti-TGFBR2 (sc-17799; Santa Cruz, Dallas, USA; 1:500, 4°C, overnight); mouse anti-ß-Actin (clone 4; MP Biomedicals, Solon, USA; 1:20,000, RT, 30 min); rabbit anti-GDF-15 (HPA011191; Sigma, Taufkirchen Germany; 1:500, 4°C, overnight); rabbit anti-phospho-Smad2 and rabbit anti-Smad2 (Ser465/467) and (86F7); Cell Signaling, Danvers, USA; 1:1000, 4°C, overnight).

Techniques: Proliferation Assay, Western Blot, Recombinant

Journal: Nature immunology

Article Title: A regulatory T cell Notch4-GDF15 axis licenses tissue inflammation in asthma

doi: 10.1038/s41590-020-0777-3

Figure Lengend Snippet: a , flow cytometric analysis and frequencies of IL13 + ILC2 (Lineage – T1/ST2 + cells) in mice of respective genotypes treated as indicated (n=5). b , c , In vitro suppression assays using ILC2 from OVA+UFP-treated Foxp3 YFPCre mice and lung T reg cells of the respective genotypes, treated as indicated (n=4) . d , GDF15 transcripts in T reg cells of Foxp3 YFPCre , Foxp3 YFPCre Notch4 Δ/Δ and Foxp3 YFPCre Ctnnb1 Δ/Δ (n=5). e , flow cytometric analysis and frequencies of GDF15 + lung T reg cells in the respective mouse genotypes treated as indicated (n=5). f , flow cytometric analysis and frequencies of IL-13 induced in naive ILC2 stimulated with IL-33, GDF15 or both (n=3). g , IL-13 expression in naive ILC2 incubated with Notch4 hi T reg cells from OVA+UFP treated mice without or with blocking GDF15 peptide (n=6). h , In vitro suppression assays using lung T reg cells and ILC2 isolated from OVA+UFP-treated Foxp3 YFPCre mice and incubated without or with GDF15 blocking peptide (n=4). Each symbol represents an independent sample. Numbers in flow plots indicate percentages. Error bars indicate SEM. Statistical tests: two-way ANOVA with Sidak’s post hoc analysis ( a - e , h ); One-way ANOVA with Dunnett’s post hoc analysis ( f,g ). *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. Data representative of two or three independent experiments.

Article Snippet: Polyclonal rabbit anti-GDF15(catalogue no: 32572–05171, 1:200, Assaypro), anti-CD16/CD32 (clone: 93, Catalogue no: 101319, 1:1000, Biolegend), Alexa Fluor 647 goat anti-rabbit IgG Ab (clone PA5–39741, catalogue no: A32733, 1:1000, Thermofischer), p-Mob1 Ab (T35; clone D2F10, Catalogue no: 8699S, 1:300, CST), rabbit anti-mouse p-Lats1 Ab (T35; clone D57D3, 1:300, CST), rabbit anti-mouse p-Lats1/2 Ab (S909/872; clone PA5–39741, catalogue no: PA5–105895, 1:300, Thermofischer), Rat anti-mouse IL-6 mAb (Clone: MP5–20F3, Catalogue no: BE0046, 1:500, Bioxcell), anti-CD28 (Clone: 37.51, Catalogue no: 122004, ,1.1000, Biolegend) , anti-IL-4 (Catalogue no: 500-P54, 1:1000, Peprotech).

Techniques: In Vitro, Expressing, Incubation, Blocking Assay, Isolation

Journal: Nature immunology

Article Title: A regulatory T cell Notch4-GDF15 axis licenses tissue inflammation in asthma

doi: 10.1038/s41590-020-0777-3

Figure Lengend Snippet: a , d , Representative PAS-stained sections of lung tissues isolated from Foxp3 YFPCre and Foxp3 YFPCre Notch4 Δ/Δ with either PBS or OVA+UFP, the latter either alone or supplemented with GDF15 or GDF15 blocking peptide, as indicated (200X magnification), Inflammation score for the respective mouse groups (n=10). b , e , AHR in Foxp3 YFPCre and Foxp3 YFPCre Notch4 Δ/Δ treated as indicated (n=10). c , f , Frequencies and absolute numbers of ILC2, eosinophils, IL-4, and IL-13, expression in lung Foxp3 – CD4 + T eff cells in the respective groups (n=10) g, AHR in Rora Cre and Rora Cre Il4/Il13 Δ/Δ treated as indicated (n=5). h, Frequencies and absolute numbers of eosinophils, ILC2, IL-4, and IL-13, expression in lung Foxp3 – CD4 + T eff cells in the respective groups (n=5). Error bars indicate SEM. Statistical tests. One-way ANOVA with Dunnett’s post hoc analysis. ( a,c,d,f ), two-way ANOVA with Sidak’s post hoc analysis ( b , e , g , h ); *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. Data representative of two or three independent experiments.

Article Snippet: Polyclonal rabbit anti-GDF15(catalogue no: 32572–05171, 1:200, Assaypro), anti-CD16/CD32 (clone: 93, Catalogue no: 101319, 1:1000, Biolegend), Alexa Fluor 647 goat anti-rabbit IgG Ab (clone PA5–39741, catalogue no: A32733, 1:1000, Thermofischer), p-Mob1 Ab (T35; clone D2F10, Catalogue no: 8699S, 1:300, CST), rabbit anti-mouse p-Lats1 Ab (T35; clone D57D3, 1:300, CST), rabbit anti-mouse p-Lats1/2 Ab (S909/872; clone PA5–39741, catalogue no: PA5–105895, 1:300, Thermofischer), Rat anti-mouse IL-6 mAb (Clone: MP5–20F3, Catalogue no: BE0046, 1:500, Bioxcell), anti-CD28 (Clone: 37.51, Catalogue no: 122004, ,1.1000, Biolegend) , anti-IL-4 (Catalogue no: 500-P54, 1:1000, Peprotech).

Techniques: Staining, Isolation, Blocking Assay, Expressing

Journal: Nature immunology

Article Title: A regulatory T cell Notch4-GDF15 axis licenses tissue inflammation in asthma

doi: 10.1038/s41590-020-0777-3

Figure Lengend Snippet: a , b , Flow cytometric analysis, cell frequencies and MFI of Notch4 expression on circulating T reg cells ( a ) and T eff cells ( b ) of control and asthmatic subjects, the latter segregated for asthma severity (control: n=39; mild n=31; moderate: n=27; severe: n=11). c , flow cytometric analysis, cell frequencies and MFI of Notch4 expression on Helios + versus Helios – circulating T reg cells of control and asthmatic subjects (control: n=13; mild n=9, moderate n=14; severe: n=11). d , e , Flow cytometric analysis, cell frequencies and MFI of Yap ( d ) and β-catenin ( e ) expression on circulating T reg cells of control and severe asthmatic subjects (control n=24; mild n=15; moderate n=15; severe: n=11). f , Serum GDF15 concentrations in moderate and severe asthmatic subjects plotted as a function of Notch4 expression on circulating T reg cells (n=21). g , In vitro suppression third party CD4 + T cells (T eff ) by the Notch4 hi versus Notch4 lo T reg cells from severe asthmatics compared to T reg cells of control subjects (n=2 subjects, 3 replicates per dilution per subject). h , In vitro suppression assays of ILC2 activation using circulating Notch4 hi T reg cells of asthmatics subjects and control T reg cells of healthy controls, incubated at the indicated T reg cell:ILC2 ratios without or with GDF15 blocking peptide (n=5). i , Flow cytometric analysis of Notch4 expression in T reg cells of a healthy control and a severe asthmatic before and after treatment with anti-IL-6R mAb (n=1). Error bars indicate SEM. Statistical tests: One-way ANOVA with Dunnett’s post hoc analysis ( a - e ); simple linear regression analysis ( f ); two-way ANOVA with Sidak’s post hoc analysis ( g,h ); ***P<0.001, ****P<0.0001. Data representative of two or three independent experiments.

Article Snippet: Polyclonal rabbit anti-GDF15(catalogue no: 32572–05171, 1:200, Assaypro), anti-CD16/CD32 (clone: 93, Catalogue no: 101319, 1:1000, Biolegend), Alexa Fluor 647 goat anti-rabbit IgG Ab (clone PA5–39741, catalogue no: A32733, 1:1000, Thermofischer), p-Mob1 Ab (T35; clone D2F10, Catalogue no: 8699S, 1:300, CST), rabbit anti-mouse p-Lats1 Ab (T35; clone D57D3, 1:300, CST), rabbit anti-mouse p-Lats1/2 Ab (S909/872; clone PA5–39741, catalogue no: PA5–105895, 1:300, Thermofischer), Rat anti-mouse IL-6 mAb (Clone: MP5–20F3, Catalogue no: BE0046, 1:500, Bioxcell), anti-CD28 (Clone: 37.51, Catalogue no: 122004, ,1.1000, Biolegend) , anti-IL-4 (Catalogue no: 500-P54, 1:1000, Peprotech).

Techniques: Expressing, In Vitro, Activation Assay, Incubation, Blocking Assay

Journal: Nature immunology

Article Title: A regulatory T cell Notch4-GDF15 axis licenses tissue inflammation in asthma

doi: 10.1038/s41590-020-0777-3

Figure Lengend Snippet: a , b , Flow cytometric analysis, cell frequencies and mean fluorescence intensity (MFI) of Notch1, 2 and 3 expression in peripheral blood T reg cells ( a ) and T eff cells ( b ) of control and asthmatic subjects, the latter segregated for asthma severity (control n=22, M.P n= 15, Mod n= 16. S.P n=11). c , Flow cytometric analysis and cell frequencies of Notch4 peripheral blood T reg cells of healthy control, food allergy (FA), eczema and FA+eczema (Control n=37, FA n= 28, Eczema n=10 and FA+Eczema n=20) d , Serum GDF15 concentrations in asthmatic subjects plotted as a function of Notch4 expression on circulating T reg cells (n=73) e , Cell frequencies of Notch4 expression in peripheral blood T reg cells in healthy subjects, allergic and non-allergic asthmatics (control = 56, non-allergic n=21, allergic n=85). Error bars indicate SEM. Statistical tests: One-way ANOVA with Dunnett’s post hoc analysis. ( a - c,e ); simple regression analysis ( d ). ***P<0.001, ****P<0.0001. Data representative of two or three independent experiments.

Article Snippet: Polyclonal rabbit anti-GDF15(catalogue no: 32572–05171, 1:200, Assaypro), anti-CD16/CD32 (clone: 93, Catalogue no: 101319, 1:1000, Biolegend), Alexa Fluor 647 goat anti-rabbit IgG Ab (clone PA5–39741, catalogue no: A32733, 1:1000, Thermofischer), p-Mob1 Ab (T35; clone D2F10, Catalogue no: 8699S, 1:300, CST), rabbit anti-mouse p-Lats1 Ab (T35; clone D57D3, 1:300, CST), rabbit anti-mouse p-Lats1/2 Ab (S909/872; clone PA5–39741, catalogue no: PA5–105895, 1:300, Thermofischer), Rat anti-mouse IL-6 mAb (Clone: MP5–20F3, Catalogue no: BE0046, 1:500, Bioxcell), anti-CD28 (Clone: 37.51, Catalogue no: 122004, ,1.1000, Biolegend) , anti-IL-4 (Catalogue no: 500-P54, 1:1000, Peprotech).

Techniques: Fluorescence, Expressing